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Abscisic acid regulates root elongation through the activities of auxin and ethylene in Arabidopsis thaliana.

Publication ,  Journal Article
Thole, JM; Beisner, ER; Liu, J; Venkova, SV; Strader, LC
Published in: G3 (Bethesda, Md.)
May 2014

Abscisic acid (ABA) regulates many aspects of plant growth and development, including inhibition of root elongation and seed germination. We performed an ABA resistance screen to identify factors required for ABA response in root elongation inhibition. We identified two classes of Arabidopsis thaliana AR mutants that displayed ABA-resistant root elongation: those that displayed resistance to ABA in both root elongation and seed germination and those that displayed resistance to ABA in root elongation but not in seed germination. We used PCR-based genotyping to identify a mutation in ABA INSENSITIVE2 (ABI2), positional information to identify mutations in AUXIN RESISTANT1 (AUX1) and ETHYLENE INSENSITIVE2 (EIN2), and whole genome sequencing to identify mutations in AUX1, AUXIN RESISTANT4 (AXR4), and ETHYLENE INSENSITIVE ROOT1/PIN-FORMED2 (EIR1/PIN2). Identification of auxin and ethylene response mutants among our isolates suggested that auxin and ethylene responsiveness were required for ABA inhibition of root elongation. To further our understanding of auxin/ethylene/ABA crosstalk, we examined ABA responsiveness of double mutants of ethylene overproducer1 (eto1) or ein2 combined with auxin-resistant mutants and found that auxin and ethylene likely operate in a linear pathway to affect ABA-responsive inhibition of root elongation, whereas these two hormones likely act independently to affect ABA-responsive inhibition of seed germination.

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Published In

G3 (Bethesda, Md.)

DOI

EISSN

2160-1836

ISSN

2160-1836

Publication Date

May 2014

Volume

4

Issue

7

Start / End Page

1259 / 1274

Related Subject Headings

  • Sequence Analysis, DNA
  • Plant Roots
  • Phenotype
  • Mutation
  • Indoleacetic Acids
  • High-Throughput Nucleotide Sequencing
  • Genotype
  • Ethylenes
  • Chromosome Mapping
  • Arabidopsis Proteins
 

Citation

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Thole, J. M., Beisner, E. R., Liu, J., Venkova, S. V., & Strader, L. C. (2014). Abscisic acid regulates root elongation through the activities of auxin and ethylene in Arabidopsis thaliana. G3 (Bethesda, Md.), 4(7), 1259–1274. https://doi.org/10.1534/g3.114.011080
Thole, Julie M., Erin R. Beisner, James Liu, Savina V. Venkova, and Lucia C. Strader. “Abscisic acid regulates root elongation through the activities of auxin and ethylene in Arabidopsis thaliana.G3 (Bethesda, Md.) 4, no. 7 (May 2014): 1259–74. https://doi.org/10.1534/g3.114.011080.
Thole JM, Beisner ER, Liu J, Venkova SV, Strader LC. Abscisic acid regulates root elongation through the activities of auxin and ethylene in Arabidopsis thaliana. G3 (Bethesda, Md). 2014 May;4(7):1259–74.
Thole, Julie M., et al. “Abscisic acid regulates root elongation through the activities of auxin and ethylene in Arabidopsis thaliana.G3 (Bethesda, Md.), vol. 4, no. 7, May 2014, pp. 1259–74. Epmc, doi:10.1534/g3.114.011080.
Thole JM, Beisner ER, Liu J, Venkova SV, Strader LC. Abscisic acid regulates root elongation through the activities of auxin and ethylene in Arabidopsis thaliana. G3 (Bethesda, Md). 2014 May;4(7):1259–1274.

Published In

G3 (Bethesda, Md.)

DOI

EISSN

2160-1836

ISSN

2160-1836

Publication Date

May 2014

Volume

4

Issue

7

Start / End Page

1259 / 1274

Related Subject Headings

  • Sequence Analysis, DNA
  • Plant Roots
  • Phenotype
  • Mutation
  • Indoleacetic Acids
  • High-Throughput Nucleotide Sequencing
  • Genotype
  • Ethylenes
  • Chromosome Mapping
  • Arabidopsis Proteins