Gateway-compatible tissue-specific vectors for plant transformation.
Journal Article (Journal Article)
Background
Understanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.Findings
To allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.Conclusions
These vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.Full Text
Duke Authors
Cited Authors
- Michniewicz, M; Frick, EM; Strader, LC
Published Date
- March 2015
Published In
Volume / Issue
- 8 /
Start / End Page
- 63 -
PubMed ID
- 25884475
Pubmed Central ID
- PMC4352289
Electronic International Standard Serial Number (EISSN)
- 1756-0500
International Standard Serial Number (ISSN)
- 1756-0500
Digital Object Identifier (DOI)
- 10.1186/s13104-015-1010-6
Language
- eng