Gateway-compatible tissue-specific vectors for plant transformation.

Journal Article (Journal Article)

Background

Understanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.

Findings

To allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.

Conclusions

These vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.

Full Text

Duke Authors

Cited Authors

  • Michniewicz, M; Frick, EM; Strader, LC

Published Date

  • March 3, 2015

Published In

Volume / Issue

  • 8 /

Start / End Page

  • 63 -

PubMed ID

  • 25884475

Pubmed Central ID

  • PMC4352289

Electronic International Standard Serial Number (EISSN)

  • 1756-0500

International Standard Serial Number (ISSN)

  • 1756-0500

Digital Object Identifier (DOI)

  • 10.1186/s13104-015-1010-6

Language

  • eng