Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos

Journal Article (Journal Article)

This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001-10 μM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from stage 10 (6-7 hpf) to 10 days post fertilization (dpf). Of the forms of mercury in this study, the organic form (MeHg) proved the most toxic followed by inorganic mercury (HgCl ), both producing embryo developmental toxicity. Altered phenotypes included pericardial edema with elongated or tube heart, reduction of eye pigmentation, and failure of swim bladder inflation. Both α-HgS and β-HgS were less toxic than MeHg and HgCl . Total RNA was extracted from survivors 3 days after exposure to MeHg (0.1 μM), HgCl (1 μM), α-HgS (10 μM), or β-HgS (10 μM) to examine toxicity-related gene expression. MeHg and HgCl markedly induced metallothionein (MT) and heme oxygenase-1 (Ho-1), while α-HgS and β-HgS failed to induce either gene. Chemical forms of mercury compounds proved to be a major determinant in their developmental toxicity. 2 2 2 2

Full Text

Duke Authors

Cited Authors

  • Dong, W; Liu, J; Wei, L; Jingfeng, Y; Chernick, M; Hinton, DE

Published Date

  • January 1, 2021

Published In

Volume / Issue

  • 4 /

Electronic International Standard Serial Number (EISSN)

  • 2167-8359

Digital Object Identifier (DOI)

  • 10.7287/peerj.preprints.2329

Citation Source

  • Scopus