The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights.

Journal Article (Journal Article)

OBJECTIVE: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. METHODS: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis-including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)-are presented. RESULTS: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid-related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. CONCLUSIONS: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.

Full Text

Duke Authors

Cited Authors

  • McCann, JR; Bihlmeyer, NA; Roche, K; Catherine, C; Jawahar, J; Kwee, LC; Younge, NE; Silverman, J; Ilkayeva, O; Sarria, C; Zizzi, A; Wootton, J; Poppe, L; Anderson, P; Arlotto, M; Wei, Z; Granek, JA; Valdivia, RH; David, LA; Dressman, HK; Newgard, CB; Shah, SH; Seed, PC; Rawls, JF; Armstrong, SC

Published Date

  • March 2021

Published In

Volume / Issue

  • 29 / 3

Start / End Page

  • 569 - 578

PubMed ID

  • 33624438

Pubmed Central ID

  • 33624438

Electronic International Standard Serial Number (EISSN)

  • 1930-739X

Digital Object Identifier (DOI)

  • 10.1002/oby.23081

Language

  • eng

Conference Location

  • United States