The nucleus is the site of transcription events - compartmentalization of transcription in eukaryotes allows for regulated access to chromatin. The nucleopore, a complex of many intrinsically disorder proteins, acts as the gatekeeper for nuclear entry and exit, and receptors for nuclear localization signals and nuclear export signals interact with both cargo and nucleopore components to facilitate this movement. Thus, regulated occlusion of the nuclear localization signal or nuclear export signal, tethering of proteins, or sequestration in biomolecular condensates can be used to regulate nucleocytoplasmic partitioning. In plants, regulated nucleocytoplasmic partitioning is a key mechanism to regulate signaling pathways, including those involved in various phytohormones, environmental stimuli, and pathogen responses.