The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective.

Journal Article (Journal Article)

Botulinum neurotoxins (BoNTs) produced by the anaerobic bacterium Clostridium botulinum are the most poisonous substances known to mankind. However, toxin regulation and signals triggering synthesis as well as the regulatory network and actors controlling toxin production are unknown. Experiments show that the neurotoxin gene is growth phase dependent for C. botulinum type A1 strain ATCC 19397, and toxin production is influenced both by culture conditions and nutritional status of the medium. Building mathematical models to describe the genetic and molecular machinery that drives the synthesis and release of BoNT requires a simultaneous description of the growth of the bacterium in culture. Here, we show four plausible modelling options which could be considered when constructing models describing the pattern of growth observed in a botulinum growth medium. Commonly used bacterial growth models are unsuitable to fit the pattern of growth observed, since they only include monotonic growth behaviour. We find that a model that includes both the nutritional status and the ability of the cells to sense their surroundings in a quorum-sensing manner is most successful at explaining the pattern of growth obtained for C. botulinum type A1 strain ATCC 19397.

Full Text

Duke Authors

Cited Authors

  • Ihekwaba, AEC; Mura, I; Peck, MW; Barker, GC

Published Date

  • December 2015

Published In

Volume / Issue

  • 73 / 9

Start / End Page

  • ftv084 -

PubMed ID

  • 26449712

Pubmed Central ID

  • PMC4622173

Electronic International Standard Serial Number (EISSN)

  • 2049-632X

International Standard Serial Number (ISSN)

  • 2049-632X

Digital Object Identifier (DOI)

  • 10.1093/femspd/ftv084

Language

  • eng