Electronic cigarette liquid exposure induces flavor-dependent osteotoxicity and increases expression of a key bone marker, collagen type I.

Journal Article (Journal Article)

Electronic cigarettes (e-cigarettes) are nicotine delivery devices advertised as a healthier alternative to conventional tobacco products, but their rapid rise in popularity outpaces research on potential health consequences. As conventional tobacco use is a risk factor for osteoporosis, this study examines whether exposure to electronic liquid (e-liquid) used in e-cigarettes affects bone-forming osteoblasts. Human MG-63 and Saos-2 osteoblast-like cells were treated for 48 hours with 0.004%-4.0% dilutions of commercially available e-liquids of various flavors with or without nicotine. Changes in cell viability and key osteoblast markers, runt-related transcription factor 2 and Col1a1, were assessed. With all e-liquids tested, cell viability decreased in a dose-dependent manner, which was least pronounced in flavorless e-liquids, most pronounced in cinnamon-flavored e-liquids and occurred independently of nicotine. Col1a1, but not runt-related transcription factor 2, mRNA expression was upregulated in response to coffee-flavored and fruit-flavored e-liquids. Cells treated with a non-cytotoxic concentration of fruit-flavored Mango Blast e-liquid with or without nicotine showed significantly increased collagen type I protein expression compared to culture medium only. We conclude that the degree of osteotoxicity is flavor-dependent and occurs independently of nicotine and that flavored e-liquids reveal collagen type I as a potential target in osteoblasts. This study elucidates potential consequences of e-cigarette use in bone.

Full Text

Duke Authors

Cited Authors

  • Otero, CE; Noeker, JA; Brown, MM; Wavreil, FDM; Harvey, WA; Mitchell, KA; Heggland, SJ

Published Date

  • June 2019

Published In

Volume / Issue

  • 39 / 6

Start / End Page

  • 888 - 898

PubMed ID

  • 30690755

Pubmed Central ID

  • PMC6531321

Electronic International Standard Serial Number (EISSN)

  • 1099-1263

International Standard Serial Number (ISSN)

  • 0260-437X

Digital Object Identifier (DOI)

  • 10.1002/jat.3777


  • eng