The transcriptional landscape of Shh medulloblastoma.

Journal Article (Journal Article)

Sonic hedgehog medulloblastoma encompasses a clinically and molecularly diverse group of cancers of the developing central nervous system. Here, we use unbiased sequencing of the transcriptome across a large cohort of 250 tumors to reveal differences among molecular subtypes of the disease, and demonstrate the previously unappreciated importance of non-coding RNA transcripts. We identify alterations within the cAMP dependent pathway (GNAS, PRKAR1A) which converge on GLI2 activity and show that 18% of tumors have a genetic event that directly targets the abundance and/or stability of MYCN. Furthermore, we discover an extensive network of fusions in focally amplified regions encompassing GLI2, and several loss-of-function fusions in tumor suppressor genes PTCH1, SUFU and NCOR1. Molecular convergence on a subset of genes by nucleotide variants, copy number aberrations, and gene fusions highlight the key roles of specific pathways in the pathogenesis of Sonic hedgehog medulloblastoma and open up opportunities for therapeutic intervention.

Full Text

Duke Authors

Cited Authors

  • Skowron, P; Farooq, H; Cavalli, FMG; Morrissy, AS; Ly, M; Hendrikse, LD; Wang, EY; Djambazian, H; Zhu, H; Mungall, KL; Trinh, QM; Zheng, T; Dai, S; Stucklin, ASG; Vladoiu, MC; Fong, V; Holgado, BL; Nor, C; Wu, X; Abd-Rabbo, D; Bérubé, P; Wang, YC; Luu, B; Suarez, RA; Rastan, A; Gillmor, AH; Lee, JJY; Zhang, XY; Daniels, C; Dirks, P; Malkin, D; Bouffet, E; Tabori, U; Loukides, J; Doz, FP; Bourdeaut, F; Delattre, OO; Masliah-Planchon, J; Ayrault, O; Kim, S-K; Meyronet, D; Grajkowska, WA; Carlotti, CG; de Torres, C; Mora, J; Eberhart, CG; Van Meir, EG; Kumabe, T; French, PJ; Kros, JM; Jabado, N; Lach, B; Pollack, IF; Hamilton, RL; Rao, AAN; Giannini, C; Olson, JM; Bognár, L; Klekner, A; Zitterbart, K; Phillips, JJ; Thompson, RC; Cooper, MK; Rubin, JB; Liau, LM; Garami, M; Hauser, P; Li, KKW; Ng, H-K; Poon, WS; Yancey Gillespie, G; Chan, JA; Jung, S; McLendon, RE; Thompson, EM; Zagzag, D; Vibhakar, R; Ra, YS; Garre, ML; Schüller, U; Shofuda, T; Faria, CC; López-Aguilar, E; Zadeh, G; Hui, C-C; Ramaswamy, V; Bailey, SD; Jones, SJ; Mungall, AJ; Moore, RA; Calarco, JA; Stein, LD; Bader, GD; Reimand, J; Ragoussis, J; Weiss, WA; Marra, MA; Suzuki, H; Taylor, MD

Published Date

  • March 19, 2021

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 1749 -

PubMed ID

  • 33741928

Pubmed Central ID

  • PMC7979819

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-021-21883-0

Language

  • eng

Conference Location

  • England