Accurate in vivo tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS).

Journal Article (Journal Article)

For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons rely on visual and physical inspection to identify tumors. Surface-enhanced Raman scattering (SERS) is emerging as a non-invasive optical alternative for intraoperative tumor identification, with high accuracy and stability. However, Raman detection requires dark rooms to work, which is not consistent with surgical settings. Methods: Herein, we used SERS nanoprobes combined with shifted-excitation Raman difference spectroscopy (SERDS) detection, to accurately detect tumors in xenograft murine model. Results: We demonstrate for the first time the use of SERDS for in vivo tumor detection in a murine model under ambient light conditions. We compare traditional Raman detection with SERDS, showing that our method can improve sensitivity and accuracy for this task. Conclusion: Our results show that this method can be used to improve the accuracy and robustness of in vivo Raman/SERS biomedical application, aiding the process of clinical translation of these technologies.

Full Text

Duke Authors

Cited Authors

  • Strobbia, P; Cupil-Garcia, V; Crawford, BM; Fales, AM; Pfefer, TJ; Liu, Y; Maiwald, M; Sumpf, B; Vo-Dinh, T

Published Date

  • January 2021

Published In

Volume / Issue

  • 11 / 9

Start / End Page

  • 4090 - 4102

PubMed ID

  • 33754050

Pubmed Central ID

  • PMC7977455

Electronic International Standard Serial Number (EISSN)

  • 1838-7640

International Standard Serial Number (ISSN)

  • 1838-7640

Digital Object Identifier (DOI)

  • 10.7150/thno.53101

Language

  • eng