Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.

Journal Article (Journal Article)

Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).

Full Text

Duke Authors

Cited Authors

  • Abutaleb, NO; Truskey, GA

Published Date

  • June 2021

Published In

Volume / Issue

  • 2 / 2

Start / End Page

  • 100394 -

PubMed ID

  • 33796871

Pubmed Central ID

  • PMC7995664

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

International Standard Serial Number (ISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2021.100394


  • eng