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Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.

Publication ,  Journal Article
Abutaleb, NO; Truskey, GA
Published in: STAR protocols
June 2021

Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).

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Published In

STAR protocols

DOI

EISSN

2666-1667

ISSN

2666-1667

Publication Date

June 2021

Volume

2

Issue

2

Start / End Page

100394

Related Subject Headings

  • Stress, Physiological
  • Stress, Mechanical
  • Induced Pluripotent Stem Cells
  • Humans
  • Endothelial Cells
  • Cells, Cultured
  • Cell Differentiation
  • Cell Culture Techniques
 

Citation

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Abutaleb, N. O., & Truskey, G. A. (2021). Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. STAR Protocols, 2(2), 100394. https://doi.org/10.1016/j.xpro.2021.100394
Abutaleb, Nadia O., and George A. Truskey. “Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.STAR Protocols 2, no. 2 (June 2021): 100394. https://doi.org/10.1016/j.xpro.2021.100394.
Abutaleb, Nadia O., and George A. Truskey. “Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.STAR Protocols, vol. 2, no. 2, June 2021, p. 100394. Epmc, doi:10.1016/j.xpro.2021.100394.
Journal cover image

Published In

STAR protocols

DOI

EISSN

2666-1667

ISSN

2666-1667

Publication Date

June 2021

Volume

2

Issue

2

Start / End Page

100394

Related Subject Headings

  • Stress, Physiological
  • Stress, Mechanical
  • Induced Pluripotent Stem Cells
  • Humans
  • Endothelial Cells
  • Cells, Cultured
  • Cell Differentiation
  • Cell Culture Techniques