In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration.

Journal Article (Journal Article)

Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here, we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa, or vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish.

Full Text

Duke Authors

Cited Authors

  • Pronobis, MI; Zheng, S; Singh, SP; Goldman, JA; Poss, KD

Published Date

  • March 25, 2021

Published In

Volume / Issue

  • 10 /

PubMed ID

  • 33764296

Pubmed Central ID

  • PMC8034980

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.66079

Language

  • eng

Conference Location

  • England