In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration.
Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here, we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa, or vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish.
Duke Scholars
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Related Subject Headings
- Zebrafish Proteins
- Zebrafish
- Signal Transduction
- Regeneration
- Myocytes, Cardiac
- Heart
- Animals, Genetically Modified
- Animals
- 42 Health sciences
- 32 Biomedical and clinical sciences
Citation
Published In
DOI
EISSN
Publication Date
Volume
Location
Related Subject Headings
- Zebrafish Proteins
- Zebrafish
- Signal Transduction
- Regeneration
- Myocytes, Cardiac
- Heart
- Animals, Genetically Modified
- Animals
- 42 Health sciences
- 32 Biomedical and clinical sciences