Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication.

Journal Article (Journal Article)

We interrogated at nucleotide resolution the spatiotemporal order of chromatin changes that occur immediately following a site-specific double-strand break (DSB) upstream of the PHO5 locus and its subsequent repair by nonhomologous end joining (NHEJ). We observed the immediate eviction of a nucleosome flanking the break and the repositioning of adjacent nucleosomes away from the break. These early chromatin events were independent of the end-processing Mre11-Rad50-Xrs2 (MRX) complex and preceded the MRX-dependent broad eviction of histones and DNA end-resectioning that extends up to ∼8 kb away from the break. We also examined the temporal dynamics of NHEJ-mediated repair in a G1-arrested population. Concomitant with DSB repair by NHEJ, we observed the redeposition and precise repositioning of nucleosomes at their originally occupied positions. This re-establishment of the prelesion chromatin landscape suggests that a DNA replication-independent mechanism exists to preserve epigenome organization following DSB repair.

Full Text

Duke Authors

Cited Authors

  • Tripuraneni, V; Memisoglu, G; MacAlpine, HK; Tran, TQ; Zhu, W; Hartemink, AJ; Haber, JE; MacAlpine, DM

Published Date

  • May 1, 2021

Published In

Volume / Issue

  • 31 / 5

Start / End Page

  • 775 - 788

PubMed ID

  • 33811083

Pubmed Central ID

  • PMC8092003

Electronic International Standard Serial Number (EISSN)

  • 1549-5469

Digital Object Identifier (DOI)

  • 10.1101/gr.271155.120

Language

  • eng

Conference Location

  • United States