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Hypoxia depletes contaminating CD45+ hematopoietic cells from murine bone marrow stromal cell (BMSC) cultures: Methods for BMSC culture purification.

Publication ,  Journal Article
Guo, W; Spiller, KV; Tang, J; Karner, CM; Hilton, MJ; Wu, C
Published in: Stem Cell Res
May 2021

Culture expanded bone marrow stromal cells (BMSCs) are easily isolated, can be grown rapidly en masse, and contain both skeletal stem cells (SSCs) and multipotent mesenchymal progenitors (MMPs). Despite this functional heterogeneity, BMSCs continue to be utilized for many applications due to the lack of definitive and universally accepted markers to prospectively identify and purify SSCs. Isolation is widely based on adherence to tissue culture plastic; however, high hematopoietic contamination is a significant impediment in murine models. Remarkably, when cultured at a physiological oxygen tension of 1% O2, a 10-fold reduction in CD45+ hematopoietic cells associated with a concomitant increase in PDGFRα+ stromal cells occur. This is due, in part, to a differential response of the two populations to hypoxia. In standard tissue culture conditions of 21% O2, CD45+ cells showed increased proliferation coupled with no changes in cell death compared to their counterparts grown at 1% O2. In contrast, PDGFR α+ stromal cells responded to hypoxia by increasing proliferation and exhibiting a 10-fold decrease in cell death. In summary, we describe a simple and reliable method exploiting the divergent biological response of hematopoietic and stromal cells to hypoxia to significantly increase the PDGFR α+ stromal cell population in murine BMSC cultures.

Duke Scholars

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Published In

Stem Cell Res

DOI

EISSN

1876-7753

Publication Date

May 2021

Volume

53

Start / End Page

102317

Location

England

Related Subject Headings

  • Stromal Cells
  • Mice
  • Mesenchymal Stem Cells
  • Hypoxia
  • Hematopoietic Stem Cell Transplantation
  • Developmental Biology
  • Cells, Cultured
  • Cell Differentiation
  • Bone Marrow Cells
  • Animals
 

Citation

APA
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ICMJE
MLA
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Guo, W., Spiller, K. V., Tang, J., Karner, C. M., Hilton, M. J., & Wu, C. (2021). Hypoxia depletes contaminating CD45+ hematopoietic cells from murine bone marrow stromal cell (BMSC) cultures: Methods for BMSC culture purification. Stem Cell Res, 53, 102317. https://doi.org/10.1016/j.scr.2021.102317
Guo, Wendi, Kassandra V. Spiller, Jackie Tang, Courtney M. Karner, Matthew J. Hilton, and Colleen Wu. “Hypoxia depletes contaminating CD45+ hematopoietic cells from murine bone marrow stromal cell (BMSC) cultures: Methods for BMSC culture purification.Stem Cell Res 53 (May 2021): 102317. https://doi.org/10.1016/j.scr.2021.102317.
Guo, Wendi, et al. “Hypoxia depletes contaminating CD45+ hematopoietic cells from murine bone marrow stromal cell (BMSC) cultures: Methods for BMSC culture purification.Stem Cell Res, vol. 53, May 2021, p. 102317. Pubmed, doi:10.1016/j.scr.2021.102317.

Published In

Stem Cell Res

DOI

EISSN

1876-7753

Publication Date

May 2021

Volume

53

Start / End Page

102317

Location

England

Related Subject Headings

  • Stromal Cells
  • Mice
  • Mesenchymal Stem Cells
  • Hypoxia
  • Hematopoietic Stem Cell Transplantation
  • Developmental Biology
  • Cells, Cultured
  • Cell Differentiation
  • Bone Marrow Cells
  • Animals