Human placental trophoblasts confer viral resistance to recipient cells.

Journal Article (Journal Article)

Placental trophoblasts form the interface between the fetal and maternal environments and serve to limit the maternal-fetal spread of viruses. Here we show that cultured primary human placental trophoblasts are highly resistant to infection by a number of viruses and, importantly, confer this resistance to nonplacental recipient cells by exosome-mediated delivery of specific microRNAs (miRNAs). We show that miRNA members of the chromosome 19 miRNA cluster, which are almost exclusively expressed in the human placenta, are packaged within trophoblast-derived exosomes and attenuate viral replication in recipient cells by the induction of autophagy. Together, our findings identify an unprecedented paracrine and/or systemic function of placental trophoblasts that uses exosome-mediated transfer of a unique set of placental-specific effector miRNAs to directly communicate with placental or maternal target cells and regulate their immunity to viral infections.

Full Text

Duke Authors

Cited Authors

  • Delorme-Axford, E; Donker, RB; Mouillet, J-F; Chu, T; Bayer, A; Ouyang, Y; Wang, T; Stolz, DB; Sarkar, SN; Morelli, AE; Sadovsky, Y; Coyne, CB

Published Date

  • July 16, 2013

Published In

Volume / Issue

  • 110 / 29

Start / End Page

  • 12048 - 12053

PubMed ID

  • 23818581

Pubmed Central ID

  • PMC3718097

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1304718110


  • eng

Conference Location

  • United States