RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections.

Journal Article (Journal Article)

It is clear that viral entry, replication, and spread is a complex process involving a dialog between the virus and the targeted host cell. Viruses have evolved highly specific strategies to hijack cellular factors to promote their internalization, initiate their replication, and facilitate their eventual spread. However, the identification of many of these host cell molecules has been hindered by the requirement for robust genome-scale loss-of-function assays that are capable of targeting a wide variety of host factors. The more recent use of genome-scale or genome-wide RNA interference (RNAi) screens have extended our knowledge of the complex interplay between a virus and host and have implicated a wide variety of cellular factors required for infection of a number of viruses. Here, we describe an approach to target mammalian host cell factors involved in regulating viral infections by the use of a genome-scale RNAi library screen.

Full Text

Duke Authors

Cited Authors

  • Coyne, CB; Cherry, S

Published Date

  • 2011

Published In

Volume / Issue

  • 721 /

Start / End Page

  • 397 - 405

PubMed ID

  • 21431700

Pubmed Central ID

  • PMC5306532

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-61779-037-9_25

Language

  • eng

Conference Location

  • United States