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Constructing an adult orofacial premotor atlas in Allen mouse CCF.

Publication ,  Journal Article
Takatoh, J; Park, JH; Lu, J; Li, S; Thompson, PM; Han, B-X; Zhao, S; Kleinfeld, D; Friedman, B; Wang, F
Published in: Elife
April 27, 2021

Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors.

Duke Scholars

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

April 27, 2021

Volume

10

Location

England

Related Subject Headings

  • Vibrissae
  • Tongue
  • Mouth
  • Motor Neurons
  • Motor Cortex
  • Mice, Inbred C57BL
  • Mice
  • Masseter Muscle
  • Male
  • Jaw
 

Citation

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Takatoh, J., Park, J. H., Lu, J., Li, S., Thompson, P. M., Han, B.-X., … Wang, F. (2021). Constructing an adult orofacial premotor atlas in Allen mouse CCF. Elife, 10. https://doi.org/10.7554/eLife.67291
Takatoh, Jun, Jae Hong Park, Jinghao Lu, Shun Li, P. M. Thompson, Bao-Xia Han, Shengli Zhao, David Kleinfeld, Beth Friedman, and Fan Wang. “Constructing an adult orofacial premotor atlas in Allen mouse CCF.Elife 10 (April 27, 2021). https://doi.org/10.7554/eLife.67291.
Takatoh J, Park JH, Lu J, Li S, Thompson PM, Han B-X, et al. Constructing an adult orofacial premotor atlas in Allen mouse CCF. Elife. 2021 Apr 27;10.
Takatoh, Jun, et al. “Constructing an adult orofacial premotor atlas in Allen mouse CCF.Elife, vol. 10, Apr. 2021. Pubmed, doi:10.7554/eLife.67291.
Takatoh J, Park JH, Lu J, Li S, Thompson PM, Han B-X, Zhao S, Kleinfeld D, Friedman B, Wang F. Constructing an adult orofacial premotor atlas in Allen mouse CCF. Elife. 2021 Apr 27;10.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

April 27, 2021

Volume

10

Location

England

Related Subject Headings

  • Vibrissae
  • Tongue
  • Mouth
  • Motor Neurons
  • Motor Cortex
  • Mice, Inbred C57BL
  • Mice
  • Masseter Muscle
  • Male
  • Jaw