Constructing an adult orofacial premotor atlas in Allen mouse CCF.

Journal Article (Journal Article)

Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors.

Full Text

Duke Authors

Cited Authors

  • Takatoh, J; Park, JH; Lu, J; Li, S; Thompson, PM; Han, B-X; Zhao, S; Kleinfeld, D; Friedman, B; Wang, F

Published Date

  • April 27, 2021

Published In

Volume / Issue

  • 10 /

PubMed ID

  • 33904410

Pubmed Central ID

  • PMC8137149

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.67291

Language

  • eng

Conference Location

  • England