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T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders.

Publication ,  Journal Article
Pulitzer, M; Geller, S; Kumar, E; Frosina, D; Moskowitz, A; Horwitz, S; Myskowski, P; Kheterpal, M; Chan, A; Dogan, A; Jungbluth, A
Published in: Histopathology
October 2018

AIMS: The diagnosis of cutaneous γδ T-cell lymphoma (GDTCL) requires the identification of γδ chains of the T-cell receptor (TCR). Our aim in this study was, by using a new monoclonal antibody (mAb) against TCRδ, to evaluate TCRδ expression in formalin-fixed paraffin-embedded (FFPE) skin tissue from TCRγ+ cutaneous T-cell lymphoma (CTCL), and to assess TCRδ expression within a spectrum of other cutaneous lymphoproliferative disorders (CLPDs). METHODS AND RESULTS: Twelve cases (10 patients) with TCRγ+ CTCL and 132 additional CLPD cases (127 patients) were examined, including mycosis fungoides (MF) (n = 60), cutaneous GDTCL (n = 15), subcutaneous panniculitis-like T-cell lymphoma (SPTCL) (n = 11), and CD30+ lymphoproliferative disorder (LPD) (n = 24). Clone H-41 against TCRδ was used on a Leica Bond-3 automated stainer to label FFPE slides. H-41 immunostaining was graded as percentage infiltrate: high (50-100%), moderate (10-49%), and low (0-9%). In TCRγ+ tumours, 12 of 12 (100%) patients showed TCRδ expression comparable to TCRγ expression. No (0%) TCRγ+ cases were negative for TCRδ. In all CLPDs, TCRδ expression was as follows: GDTCL, 16 of 20 cases (14 of 15 patients) high, two moderate, and two low; MF, 0 of 60 cases high, nine moderate, and 51 low; CD30+ LPD, one of 24 cases high, two moderate, and 21 low; and SPTCL, 0 of 11 cases (0 of 9 patients) high, two moderate, and two low. Three MF-like cases and one SPTCL-like case showed high expression; the remainder showed low expression. CONCLUSIONS: mAb H-41 against TCRδ matches TCRγ in immunostaining FFPE tissues from GDTCL, supporting H-41 as a replacement for mAb γ3.20. TCRδ expression in our study suggests that the true occurrence of γδ+ non-GDTCL CTCL/CLPD may be lower than suggested by the recent literature.

Duke Scholars

Published In

Histopathology

DOI

EISSN

1365-2559

Publication Date

October 2018

Volume

73

Issue

4

Start / End Page

653 / 662

Location

England

Related Subject Headings

  • Skin Neoplasms
  • Receptors, Antigen, T-Cell, gamma-delta
  • Pathology
  • Male
  • Lymphoproliferative Disorders
  • Lymphoma, T-Cell, Cutaneous
  • Immunohistochemistry
  • Humans
  • Female
  • Antibodies, Monoclonal
 

Citation

APA
Chicago
ICMJE
MLA
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Pulitzer, M., Geller, S., Kumar, E., Frosina, D., Moskowitz, A., Horwitz, S., … Jungbluth, A. (2018). T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders. Histopathology, 73(4), 653–662. https://doi.org/10.1111/his.13671
Pulitzer, Melissa, Shamir Geller, Erica Kumar, Denise Frosina, Alison Moskowitz, Steven Horwitz, Patricia Myskowski, et al. “T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders.Histopathology 73, no. 4 (October 2018): 653–62. https://doi.org/10.1111/his.13671.
Pulitzer M, Geller S, Kumar E, Frosina D, Moskowitz A, Horwitz S, et al. T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders. Histopathology. 2018 Oct;73(4):653–62.
Pulitzer, Melissa, et al. “T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders.Histopathology, vol. 73, no. 4, Oct. 2018, pp. 653–62. Pubmed, doi:10.1111/his.13671.
Pulitzer M, Geller S, Kumar E, Frosina D, Moskowitz A, Horwitz S, Myskowski P, Kheterpal M, Chan A, Dogan A, Jungbluth A. T-cell receptor-δ expression and γδ+ T-cell infiltrates in primary cutaneous γδ T-cell lymphoma and other cutaneous T-cell lymphoproliferative disorders. Histopathology. 2018 Oct;73(4):653–662.
Journal cover image

Published In

Histopathology

DOI

EISSN

1365-2559

Publication Date

October 2018

Volume

73

Issue

4

Start / End Page

653 / 662

Location

England

Related Subject Headings

  • Skin Neoplasms
  • Receptors, Antigen, T-Cell, gamma-delta
  • Pathology
  • Male
  • Lymphoproliferative Disorders
  • Lymphoma, T-Cell, Cutaneous
  • Immunohistochemistry
  • Humans
  • Female
  • Antibodies, Monoclonal