Using BioID to Characterize the RAS Interactome.

Journal Article (Journal Article)

Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.

Full Text

Duke Authors

Cited Authors

  • Adhikari, H; Counter, CM

Published Date

  • 2021

Published In

Volume / Issue

  • 2262 /

Start / End Page

  • 271 - 280

PubMed ID

  • 33977483

Pubmed Central ID

  • PMC8251342

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1190-6_16

Language

  • eng

Conference Location

  • United States