FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species.

Journal Article (Journal Article)

Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.

Full Text

Duke Authors

Cited Authors

  • Song, Y; Wilson, AJ; Zhang, X-C; Thoms, D; Sohrabi, R; Song, S; Geissmann, Q; Liu, Y; Walgren, L; He, SY; Haney, CH

Published Date

  • May 10, 2021

Published In

Volume / Issue

  • 7 / 5

Start / End Page

  • 644 - 654

PubMed ID

  • 33972713

Electronic International Standard Serial Number (EISSN)

  • 2055-0278

International Standard Serial Number (ISSN)

  • 2055-0278

Digital Object Identifier (DOI)

  • 10.1038/s41477-021-00914-0

Language

  • eng