Skip to main content

Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy.

Publication ,  Journal Article
Zhang, C; Welsher, K
Published in: Entropy (Basel, Switzerland)
April 2021

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Entropy (Basel, Switzerland)

DOI

EISSN

1099-4300

ISSN

1099-4300

Publication Date

April 2021

Volume

23

Issue

5

Start / End Page

498

Related Subject Headings

  • Fluids & Plasmas
  • 51 Physical sciences
  • 49 Mathematical sciences
  • 02 Physical Sciences
  • 01 Mathematical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, C., & Welsher, K. (2021). Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy. Entropy (Basel, Switzerland), 23(5), 498. https://doi.org/10.3390/e23050498
Zhang, Chen, and Kevin Welsher. “Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy.Entropy (Basel, Switzerland) 23, no. 5 (April 2021): 498. https://doi.org/10.3390/e23050498.
Zhang, Chen, and Kevin Welsher. “Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy.Entropy (Basel, Switzerland), vol. 23, no. 5, Apr. 2021, p. 498. Epmc, doi:10.3390/e23050498.

Published In

Entropy (Basel, Switzerland)

DOI

EISSN

1099-4300

ISSN

1099-4300

Publication Date

April 2021

Volume

23

Issue

5

Start / End Page

498

Related Subject Headings

  • Fluids & Plasmas
  • 51 Physical sciences
  • 49 Mathematical sciences
  • 02 Physical Sciences
  • 01 Mathematical Sciences