Early-life mitochondrial DNA damage results in lifelong deficits in energy production mediated by redox signaling in Caenorhabditis elegans.

Journal Article (Journal Article)

The consequences of damage to the mitochondrial genome (mtDNA) are poorly understood, although mtDNA is more susceptible to damage resulting from some genotoxicants than nuclear DNA (nucDNA), and many environmental toxicants target the mitochondria. Reports from the toxicological literature suggest that exposure to early-life mitochondrial damage could lead to deleterious consequences later in life (the "Developmental Origins of Health and Disease" paradigm), but reports from other fields often report beneficial ("mitohormetic") responses to such damage. Here, we tested the effects of low (causing no change in lifespan) levels of ultraviolet C (UVC)-induced, irreparable mtDNA damage during early development in Caenorhabditis elegans. This exposure led to life-long reductions in mtDNA copy number and steady-state ATP levels, accompanied by increased oxygen consumption and altered metabolite profiles, suggesting inefficient mitochondrial function. Exposed nematodes were also developmentally delayed, reached smaller adult size, and were rendered more susceptible to subsequent exposure to chemical mitotoxicants. Metabolomic and genetic analysis of key signaling and metabolic pathways supported redox and mitochondrial stress-response signaling during early development as a mechanism for establishing these persistent alterations. Our results highlight the importance of early-life exposures to environmental pollutants, especially in the context of exposure to chemicals that target mitochondria.

Full Text

Duke Authors

Cited Authors

  • Hershberger, KA; Rooney, JP; Turner, EA; Donoghue, LJ; Bodhicharla, R; Maurer, LL; Ryde, IT; Kim, JJ; Joglekar, R; Hibshman, JD; Smith, LL; Bhatt, DP; Ilkayeva, OR; Hirschey, MD; Meyer, JN

Published Date

  • July 2021

Published In

Volume / Issue

  • 43 /

Start / End Page

  • 102000 -

PubMed ID

  • 33993056

Pubmed Central ID

  • PMC8134077

Electronic International Standard Serial Number (EISSN)

  • 2213-2317

Digital Object Identifier (DOI)

  • 10.1016/j.redox.2021.102000

Language

  • eng

Conference Location

  • Netherlands