Comparative evaluation of the microbicidal activity of low-temperature sterilization technologies to steam sterilization.

Journal Article (Journal Article)

OBJECTIVE: To compare the microbicidal activity of low-temperature sterilization technologies (vaporized hydrogen peroxide [VHP], ethylene oxide [ETO], and hydrogen peroxide gas plasma [HPGP]) to steam sterilization in the presence of salt and serum to simulate inadequate precleaning. METHODS: Test carriers were inoculated with Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, vancomycin-resistant Enterococcus, Mycobacterium terrae, Bacillus atrophaeus spores, Geobacillus stearothermophilus spores, or Clostridiodes difficile spores in the presence of salt and serum and then subjected to 4 sterilization technologies: steam, ETO, VHP and HPGP. RESULTS: Steam, ETO, and HPGP sterilization techniques were capable of inactivating the test organisms on stainless steel carriers with a failure rate of 0% (0 of 220), 1.9% (6 of 310), and 1.9% (5 of 270), respectively. The failure rate for VHP was 76.3% (206 of 270). CONCLUSION: Steam sterilization is the most effective and had the largest margin of safety, followed by ETO and HPGP, but VHP showed much less efficacy.

Full Text

Duke Authors

Cited Authors

  • Rutala, WA; Gergen, MF; Sickbert-Bennett, EE; Weber, DJ

Published Date

  • April 2020

Published In

Volume / Issue

  • 41 / 4

Start / End Page

  • 391 - 395

PubMed ID

  • 32098638

Electronic International Standard Serial Number (EISSN)

  • 1559-6834

Digital Object Identifier (DOI)

  • 10.1017/ice.2020.2

Language

  • eng

Conference Location

  • United States