Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing.
Journal Article (Journal Article)
Epitranscriptomic RNA modifications function as an important layer of gene regulation that modulates the function of RNA transcripts. A key step in understanding how RNA modifications regulate biological processes is the mapping of their locations, which is most commonly done by RNA immunoprecipitation (RIP) using modification-specific antibodies. Here, we describe the use of a photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP) method, in conjunction with RNA modification-specific antibodies, to map modification sites. First described as photo-crosslinking-assisted m6A sequencing (PA-m6A-seq), this method allows the mapping of RNA modifications at a higher resolution, with lower background than traditional RIP, and can be adapted to any RNA modification for which a specific antibody is available.
Full Text
Duke Authors
Cited Authors
- Cullen, BR; Tsai, K
Published Date
- 2021
Published In
Volume / Issue
- 2298 /
Start / End Page
- 123 - 134
PubMed ID
- 34085242
Pubmed Central ID
- PMC8259884
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/978-1-0716-1374-0_8
Language
- eng
Conference Location
- United States