Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing.

Journal Article (Journal Article)

Epitranscriptomic RNA modifications function as an important layer of gene regulation that modulates the function of RNA transcripts. A key step in understanding how RNA modifications regulate biological processes is the mapping of their locations, which is most commonly done by RNA immunoprecipitation (RIP) using modification-specific antibodies. Here, we describe the use of a photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP) method, in conjunction with RNA modification-specific antibodies, to map modification sites. First described as photo-crosslinking-assisted m6A sequencing (PA-m6A-seq), this method allows the mapping of RNA modifications at a higher resolution, with lower background than traditional RIP, and can be adapted to any RNA modification for which a specific antibody is available.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR; Tsai, K

Published Date

  • 2021

Published In

Volume / Issue

  • 2298 /

Start / End Page

  • 123 - 134

PubMed ID

  • 34085242

Pubmed Central ID

  • PMC8259884

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1374-0_8

Language

  • eng

Conference Location

  • United States