PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.

Journal Article (Journal Article)

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for simultaneous isolation of mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material, and availability of specific PCR reagents. We also briefly describe factors that affect mtDNA copy number and discuss caveats to its use as a biomarker.

Full Text

Duke Authors

Cited Authors

  • Leuthner, TC; Hartman, JH; Ryde, IT; Meyer, JN

Published Date

  • January 2021

Published In

Volume / Issue

  • 2310 /

Start / End Page

  • 91 - 111

PubMed ID

  • 34096001

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1433-4_8

Language

  • eng