Genome sequencing unveils a regulatory landscape of platelet reactivity.

Journal Article (Journal Article)

Platelet aggregation at the site of atherosclerotic vascular injury is the underlying pathophysiology of myocardial infarction and stroke. To build upon prior GWAS, here we report on 16 loci identified through a whole genome sequencing (WGS) approach in 3,855 NHLBI Trans-Omics for Precision Medicine (TOPMed) participants deeply phenotyped for platelet aggregation. We identify the RGS18 locus, which encodes a myeloerythroid lineage-specific regulator of G-protein signaling that co-localizes with expression quantitative trait loci (eQTL) signatures for RGS18 expression in platelets. Gene-based approaches implicate the SVEP1 gene, a known contributor of coronary artery disease risk. Sentinel variants at RGS18 and PEAR1 are associated with thrombosis risk and increased gastrointestinal bleeding risk, respectively. Our WGS findings add to previously identified GWAS loci, provide insights regarding the mechanism(s) by which genetics may influence cardiovascular disease risk, and underscore the importance of rare variant and regulatory approaches to identifying loci contributing to complex phenotypes.

Full Text

Duke Authors

Cited Authors

  • Keramati, AR; Chen, M-H; Rodriguez, BAT; Yanek, LR; Bhan, A; Gaynor, BJ; Ryan, K; Brody, JA; Zhong, X; Wei, Q; NHLBI Trans-Omics for Precision (TOPMed) Consortium, ; Kammers, K; Kanchan, K; Iyer, K; Kowalski, MH; Pitsillides, AN; Cupples, LA; Li, B; Schlaeger, TM; Shuldiner, AR; O'Connell, JR; Ruczinski, I; Mitchell, BD; Faraday, N; Taub, MA; Becker, LC; Lewis, JP; Mathias, RA; Johnson, AD

Published Date

  • June 15, 2021

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 3626 -

PubMed ID

  • 34131117

Pubmed Central ID

  • PMC8206369

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-021-23470-9

Language

  • eng

Conference Location

  • England