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Microscope-Integrated OCT-Guided Volumetric Measurements of Subretinal Blebs Created by a Suprachoroidal Approach.

Publication ,  Journal Article
Sastry, A; Li, JD; Raynor, W; Viehland, C; Song, Z; Xu, L; Farsiu, S; Izatt, JA; Toth, CA; Vajzovic, L
Published in: Transl Vis Sci Technol
June 1, 2021

PURPOSE: To investigate the use of imaging modalities in the volumetric measurement of the subretinal space and examine the volume of subretinal blebs created by a subretinal drug delivery device utilizing microscope-integrated optical coherence tomography (MIOCT). METHODS: An MIOCT image-based volume measurement method was developed and assessed for accuracy and reproducibility by imaging ceramic spheres of known size that were surgically implanted into ex vivo porcine eyes. This method was then used to measure subretinal blebs created in 10 porcine eyes by injection of balanced salt solution utilizing a subretinal delivery device via a suprachoroidal cannula. Bleb volumes obtained from MIOCT were compared to the intended injection volume. RESULTS: Validation of image-based volume measurements of ceramic spheres showed accuracy to ±0.029 µL (5.6%) for objects imaged over the posterior pole and ±0.025 µL (4.8%) over peripheral retina. The mean expected injection volume from extraocular tests of the suprachoroidal cannula was 66.44 µL (σ = 2.4 µL). The mean injection volume as measured by the MIOCT imaging method was 54.8 µL (σ = 12.3 µL), or 82.48% of expected injection volume. CONCLUSIONS: MIOCT can measure the volume of subretinal blebs with accuracy and precision. The novel suprachoroidal approach using a subretinal delivery device was able to deliver greater than 80% of expected injection volume into the subretinal space, as assessed by MIOCT. TRANSLATIONAL RELEVANCE: MIOCT provides a method for visualization, and analysis of images enables surgeons to quantify and evaluate the success of subretinal drug delivery via a suprachoroidal approach.

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Published In

Transl Vis Sci Technol

DOI

EISSN

2164-2591

Publication Date

June 1, 2021

Volume

10

Issue

7

Start / End Page

24

Location

United States

Related Subject Headings

  • Tomography, Optical Coherence
  • Swine
  • Retina
  • Reproducibility of Results
  • Animals
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry
  • 0903 Biomedical Engineering
 

Citation

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Sastry, A., Li, J. D., Raynor, W., Viehland, C., Song, Z., Xu, L., … Vajzovic, L. (2021). Microscope-Integrated OCT-Guided Volumetric Measurements of Subretinal Blebs Created by a Suprachoroidal Approach. Transl Vis Sci Technol, 10(7), 24. https://doi.org/10.1167/tvst.10.7.24
Sastry, Ananth, Jianwei D. Li, William Raynor, Christian Viehland, Zhenxi Song, Liangyu Xu, Sina Farsiu, Joseph A. Izatt, Cynthia A. Toth, and Lejla Vajzovic. “Microscope-Integrated OCT-Guided Volumetric Measurements of Subretinal Blebs Created by a Suprachoroidal Approach.Transl Vis Sci Technol 10, no. 7 (June 1, 2021): 24. https://doi.org/10.1167/tvst.10.7.24.
Sastry A, Li JD, Raynor W, Viehland C, Song Z, Xu L, et al. Microscope-Integrated OCT-Guided Volumetric Measurements of Subretinal Blebs Created by a Suprachoroidal Approach. Transl Vis Sci Technol. 2021 Jun 1;10(7):24.
Sastry, Ananth, et al. “Microscope-Integrated OCT-Guided Volumetric Measurements of Subretinal Blebs Created by a Suprachoroidal Approach.Transl Vis Sci Technol, vol. 10, no. 7, June 2021, p. 24. Pubmed, doi:10.1167/tvst.10.7.24.
Sastry A, Li JD, Raynor W, Viehland C, Song Z, Xu L, Farsiu S, Izatt JA, Toth CA, Vajzovic L. Microscope-Integrated OCT-Guided Volumetric Measurements of Subretinal Blebs Created by a Suprachoroidal Approach. Transl Vis Sci Technol. 2021 Jun 1;10(7):24.

Published In

Transl Vis Sci Technol

DOI

EISSN

2164-2591

Publication Date

June 1, 2021

Volume

10

Issue

7

Start / End Page

24

Location

United States

Related Subject Headings

  • Tomography, Optical Coherence
  • Swine
  • Retina
  • Reproducibility of Results
  • Animals
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry
  • 0903 Biomedical Engineering