PLGA nanoparticle entrapped killed porcine reproductive and respiratory syndrome virus vaccine helps in viral clearance in pigs.

Journal Article (Journal Article)

Porcine reproductive and respiratory syndrome (PRRS) is a chronic viral disease of pigs, has been posing a huge economic concern to pig industry worldwide. In this study, we developed biodegradable PLGA [poly(d,l-lactide-co-glycolide)] nanoparticle-entrapped killed PRRSV vaccine (Nano-KAg), and administered intranasally to pigs once and evaluated the immune correlates. In Nano-KAg vaccinated homologous virus challenged pigs, complete clearance of viremia was observed in 2 weeks, associated with a significant increase in virus neutralizing titers only in the lungs, compared to both unvaccinated and killed vaccine vaccinated pigs. The lung homogenate and sera of Nano-KAg vaccinated pigs had higher levels of IFN-γ and lower levels of TGF-β than control groups. Restimulation of mononuclear cells isolated from the lungs, blood, BAL, and TBLN of Nano-KAg vaccinated pigs' secreted significantly increased levels of Th1 cytokines, IFN-γ and IL-12. In addition, higher frequencies of CD3(+)CD8(+), CD4(+)CD8(+), and γδ T cells, and reduced frequency of Foxp3(+) T-regulatory cells were observed in Nano-KAg vaccinated pigs. Thus, intranasal delivery of Nano-KAg vaccine may be a suitable strategy to elicit anti-PRRSV immune response required to better clear viremia in pigs.

Full Text

Duke Authors

Cited Authors

  • Dwivedi, V; Manickam, C; Binjawadagi, B; Renukaradhya, GJ

Published Date

  • September 27, 2013

Published In

Volume / Issue

  • 166 / 1-2

Start / End Page

  • 47 - 58

PubMed ID

  • 23764272

Pubmed Central ID

  • PMC7117126

Electronic International Standard Serial Number (EISSN)

  • 1873-2542

Digital Object Identifier (DOI)

  • 10.1016/j.vetmic.2013.04.029

Language

  • eng

Conference Location

  • Netherlands