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Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs.

Publication ,  Journal Article
Manickam, C; Dwivedi, V; Miller, J; Papenfuss, T; Renukaradhya, GJ
Published in: Viral Immunol
February 2013

Porcine respiratory and reproductive syndrome (PRRS) is an economically important disease of pigs worldwide. Currently used PRRSV vaccines provide incomplete protection. Recently, we identified Mycobacterium tuberculosis whole cell lysate (Mtb WCL) as a potent mucosal adjuvant to modified live PRRSV vaccine (PRRS-MLV). In this study, pigs were unvaccinated or vaccinated with PRRS-MLV plus Mtb WCL, intranasally, and challenged with either homologous (strain VR2332) or virulent heterologous (strain MN184) PRRSV; subsequently, euthanized at three time points post-challenge to evaluate lung immune responses. Microscopic examination of lung sections revealed reduced disruption of the lung architecture and less of interstitial pneumonia in vaccinated, compared to unvaccinated MN184 challenged pigs. The restimulated lung and peripheral blood mononuclear cells revealed increased proliferation of CD8(+) lymphocytes, and in the lung homogenate increased secretion of nitric oxide was detected in vaccinated MN184 challenged pigs. In summary, the adjuvant effects of Mtb WCL to PRRS-MLV resulted in favorable anti-PRRSV immune microenvironment in the lungs to help better viral clearance.

Duke Scholars

Published In

Viral Immunol

DOI

EISSN

1557-8976

Publication Date

February 2013

Volume

26

Issue

1

Start / End Page

102 / 108

Location

United States

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Swine
  • Porcine respiratory and reproductive syndrome virus
  • Porcine Reproductive and Respiratory Syndrome
  • Nitric Oxide
  • Mycobacterium tuberculosis
  • Lung Diseases, Interstitial
  • Lung
  • Leukocytes, Mononuclear
 

Citation

APA
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ICMJE
MLA
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Manickam, C., Dwivedi, V., Miller, J., Papenfuss, T., & Renukaradhya, G. J. (2013). Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs. Viral Immunol, 26(1), 102–108. https://doi.org/10.1089/vim.2012.0065
Manickam, Cordelia, Varun Dwivedi, Jayla Miller, Tracey Papenfuss, and Gourapura J. Renukaradhya. “Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs.Viral Immunol 26, no. 1 (February 2013): 102–8. https://doi.org/10.1089/vim.2012.0065.
Journal cover image

Published In

Viral Immunol

DOI

EISSN

1557-8976

Publication Date

February 2013

Volume

26

Issue

1

Start / End Page

102 / 108

Location

United States

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Swine
  • Porcine respiratory and reproductive syndrome virus
  • Porcine Reproductive and Respiratory Syndrome
  • Nitric Oxide
  • Mycobacterium tuberculosis
  • Lung Diseases, Interstitial
  • Lung
  • Leukocytes, Mononuclear