Functional invariant NKT cells in pig lungs regulate the airway hyperreactivity: a potential animal model.
Journal Article (Journal Article)
Important roles played by invariant natural killer T (iNKT) cells in asthma pathogenesis have been demonstrated. We identified functional iNKT cells and CD1d molecules in pig lungs. Pig iNKT cells cultured in the presence of α-GalCer proliferated and secreted Th1 and Th2 cytokines. Like in other animal models, direct activation of pig lung iNKT cells using α-GalCer resulted in acute airway hyperreactivity (AHR). Clinically, acute AHR-induced pigs had increased respiratory rate, enhanced mucus secretion in the airways, fever, etc. In addition, we observed petechial hemorrhages, infiltration of CD4(+) cells, and increased Th2 cytokines in AHR-induced pig lungs. Ex vivo proliferated iNKT cells of asthma induced pigs in the presence of C-glycoside analogs of α-GalCer had predominant Th2 phenotype and secreted more of Th2 cytokine, IL-4. Thus, baby pigs may serve as a useful animal model to study iNKT cell-mediated AHR caused by various environmental and microbial CD1d-specific glycolipid antigens.
Full Text
Duke Authors
Cited Authors
- Renukaradhya, GJ; Manickam, C; Khatri, M; Rauf, A; Li, X; Tsuji, M; Rajashekara, G; Dwivedi, V
Published Date
- April 2011
Published In
Volume / Issue
- 31 / 2
Start / End Page
- 228 - 239
PubMed ID
- 21042929
Pubmed Central ID
- PMC4450678
Electronic International Standard Serial Number (EISSN)
- 1573-2592
Digital Object Identifier (DOI)
- 10.1007/s10875-010-9476-4
Language
- eng
Conference Location
- Netherlands