Functional invariant NKT cells in pig lungs regulate the airway hyperreactivity: a potential animal model.

Journal Article (Journal Article)

Important roles played by invariant natural killer T (iNKT) cells in asthma pathogenesis have been demonstrated. We identified functional iNKT cells and CD1d molecules in pig lungs. Pig iNKT cells cultured in the presence of α-GalCer proliferated and secreted Th1 and Th2 cytokines. Like in other animal models, direct activation of pig lung iNKT cells using α-GalCer resulted in acute airway hyperreactivity (AHR). Clinically, acute AHR-induced pigs had increased respiratory rate, enhanced mucus secretion in the airways, fever, etc. In addition, we observed petechial hemorrhages, infiltration of CD4(+) cells, and increased Th2 cytokines in AHR-induced pig lungs. Ex vivo proliferated iNKT cells of asthma induced pigs in the presence of C-glycoside analogs of α-GalCer had predominant Th2 phenotype and secreted more of Th2 cytokine, IL-4. Thus, baby pigs may serve as a useful animal model to study iNKT cell-mediated AHR caused by various environmental and microbial CD1d-specific glycolipid antigens.

Full Text

Duke Authors

Cited Authors

  • Renukaradhya, GJ; Manickam, C; Khatri, M; Rauf, A; Li, X; Tsuji, M; Rajashekara, G; Dwivedi, V

Published Date

  • April 2011

Published In

Volume / Issue

  • 31 / 2

Start / End Page

  • 228 - 239

PubMed ID

  • 21042929

Pubmed Central ID

  • PMC4450678

Electronic International Standard Serial Number (EISSN)

  • 1573-2592

Digital Object Identifier (DOI)

  • 10.1007/s10875-010-9476-4

Language

  • eng

Conference Location

  • Netherlands