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Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes

Publication ,  Journal Article
Wang, Y; Chen, Y; Xin, L; Beverley, SM; Carlsen, ED; Popov, V; Chang, K-P; Wang, M; Soong, L
Published in: Infection and Immunity
March 2011

Recent studies have shown that histone proteins can act as antimicrobial peptides in host defense against extracellular bacteria, fungi, and promastigotes. In this study, we used human recombinant histone proteins to further study their leishmaniacidal effects and the underlying mechanisms. We found that the histones H2A and H2B (but not H1 ) could directly and efficiently kill promastigotes of , , , and in a treatment dose-dependent manner. Scanning electron microscopy revealed surface disruption of histone-treated promastigotes. More importantly, the preexposure of promastigotes to histone proteins markedly decreased the infectivity of promastigotes to murine macrophages (Mφs) . However, axenic and lesion-derived amastigotes of and were relatively resistant to histone treatment, which correlated with the low levels of intracellular H2A in treated amastigotes. To understand the mechanisms underlying these differential responses, we investigated the role of promastigote surface molecules in histone-mediated killing. Compared with the corresponding controls, transgenic promastigotes expressing lower levels of surface gp63 proteins were more susceptible to histone H2A, while and promastigotes with targeted deletion of the lipophosphoglycan 2 ( ) gene (but not the gene) were more resistant to histone H2A. We discuss the influence of promastigote major surface molecules in the leishmaniacidal effect of histone proteins. This study provides new information on host innate immunity to different developmental stages of parasites.

Duke Scholars

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Published In

Infection and Immunity

DOI

EISSN

1098-5522

ISSN

0019-9567

Publication Date

March 2011

Volume

79

Issue

3

Start / End Page

1124 / 1133

Publisher

American Society for Microbiology

Related Subject Headings

  • Microbiology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

Citation

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Wang, Y., Chen, Y., Xin, L., Beverley, S. M., Carlsen, E. D., Popov, V., … Soong, L. (2011). Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes. Infection and Immunity, 79(3), 1124–1133. https://doi.org/10.1128/iai.00658-10
Wang, Yingwei, Yang Chen, Lijun Xin, Stephen M. Beverley, Eric D. Carlsen, Vsevolod Popov, Kwang-Poo Chang, Ming Wang, and Lynn Soong. “Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes.” Edited by J. F. Urban. Infection and Immunity 79, no. 3 (March 2011): 1124–33. https://doi.org/10.1128/iai.00658-10.
Wang Y, Chen Y, Xin L, Beverley SM, Carlsen ED, Popov V, et al. Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes. Urban JF, editor. Infection and Immunity. 2011 Mar;79(3):1124–33.
Wang, Yingwei, et al. “Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes.” Infection and Immunity, edited by J. F. Urban, vol. 79, no. 3, American Society for Microbiology, Mar. 2011, pp. 1124–33. Crossref, doi:10.1128/iai.00658-10.
Wang Y, Chen Y, Xin L, Beverley SM, Carlsen ED, Popov V, Chang K-P, Wang M, Soong L. Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes. Urban JF, editor. Infection and Immunity. American Society for Microbiology; 2011 Mar;79(3):1124–1133.

Published In

Infection and Immunity

DOI

EISSN

1098-5522

ISSN

0019-9567

Publication Date

March 2011

Volume

79

Issue

3

Start / End Page

1124 / 1133

Publisher

American Society for Microbiology

Related Subject Headings

  • Microbiology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences