A comprehensive model to predict mitotic division in budding yeasts.

Journal Article (Journal Article)

High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division.

Full Text

Duke Authors

Cited Authors

  • Sutradhar, S; Yadav, V; Sridhar, S; Sreekumar, L; Bhattacharyya, D; Ghosh, SK; Paul, R; Sanyal, K

Published Date

  • November 2015

Published In

Volume / Issue

  • 26 / 22

Start / End Page

  • 3954 - 3965

PubMed ID

  • 26310442

Pubmed Central ID

  • PMC4710229

Electronic International Standard Serial Number (EISSN)

  • 1939-4586

International Standard Serial Number (ISSN)

  • 1059-1524

Digital Object Identifier (DOI)

  • 10.1091/mbc.e15-04-0236


  • eng