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Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test.

Publication ,  Journal Article
Heggestad, JT; Kinnamon, DS; Olson, LB; Liu, J; Kelly, G; Wall, SA; Fontes, CM; Joh, DY; Hucknall, AM; Pieper, C; Naqvi, IA; Chen, L; Que, LG ...
Published in: medRxiv
November 7, 2020

Highly sensitive, specific, and point-of-care (POC) serological assays are an essential tool to manage the COVID-19 pandemic. Here, we report on a microfluidic, multiplexed POC test that can profile the antibody response against multiple SARS-CoV-2 antigens - Spike S1 (S1), Nucleocapsid (N), and the receptor binding domain (RBD) - simultaneously from a 60 microliter drop of blood, plasma, or serum. We assessed the levels of anti-SARS-CoV-2 antibodies in plasma samples from 19 individuals (at multiple time points) with COVID-19 that required admission to the intensive care unit and from 10 healthy individuals. This POC assay shows good concordance with a live virus microneutralization assay, achieved high sensitivity (100%) and specificity (100%), and successfully tracked the longitudinal evolution of the antibody response in infected individuals. We also demonstrated that we can detect a chemokine, IP-10, on the same chip, which may provide prognostic insight into patient outcomes. Because our test requires minimal user intervention and is read by a handheld detector, it can be globally deployed in the fight against COVID-19 by democratizing access to laboratory quality tests.

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medRxiv

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Publication Date

November 7, 2020

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United States
 

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Heggestad, J. T., Kinnamon, D. S., Olson, L. B., Liu, J., Kelly, G., Wall, S. A., … Chilkoti, A. (2020). Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test. MedRxiv. https://doi.org/10.1101/2020.11.05.20226654
Heggestad, Jacob T., David S. Kinnamon, Lyra B. Olson, Jason Liu, Garrett Kelly, Simone A. Wall, Cassio M. Fontes, et al. “Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test.MedRxiv, November 7, 2020. https://doi.org/10.1101/2020.11.05.20226654.
Heggestad JT, Kinnamon DS, Olson LB, Liu J, Kelly G, Wall SA, et al. Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test. medRxiv. 2020 Nov 7;
Heggestad, Jacob T., et al. “Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test.MedRxiv, Nov. 2020. Pubmed, doi:10.1101/2020.11.05.20226654.
Heggestad JT, Kinnamon DS, Olson LB, Liu J, Kelly G, Wall SA, Fontes CM, Joh DY, Hucknall AM, Pieper C, Naqvi IA, Chen L, Que LG, Oguin T, Nair SK, Sullenger BA, Woods CW, Sempowski GD, Kraft BD, Chilkoti A. Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test. medRxiv. 2020 Nov 7;

Published In

medRxiv

DOI

Publication Date

November 7, 2020

Location

United States