Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity.

Journal Article (Journal Article)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with multiple spike mutations enable increased transmission and antibody resistance. We combined cryo-electron microscopy (cryo-EM), binding, and computational analyses to study variant spikes, including one that was involved in transmission between minks and humans, and others that originated and spread in human populations. All variants showed increased angiotensin-converting enzyme 2 (ACE2) receptor binding and increased propensity for receptor binding domain (RBD)-up states. While adaptation to mink resulted in spike destabilization, the B.1.1.7 (UK) spike balanced stabilizing and destabilizing mutations. A local destabilizing effect of the RBD E484K mutation was implicated in resistance of the B.1.1.28/P.1 (Brazil) and B.1.351 (South Africa) variants to neutralizing antibodies. Our studies revealed allosteric effects of mutations and mechanistic differences that drive either interspecies transmission or escape from antibody neutralization.

Full Text

Duke Authors

Cited Authors

  • Gobeil, SM-C; Janowska, K; McDowell, S; Mansouri, K; Parks, R; Stalls, V; Kopp, MF; Manne, K; Li, D; Wiehe, K; Saunders, KO; Edwards, RJ; Korber, B; Haynes, BF; Henderson, R; Acharya, P

Published Date

  • August 6, 2021

Published In

Volume / Issue

  • 373 / 6555

PubMed ID

  • 34168071

Pubmed Central ID

  • PMC8611377

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.abi6226

Language

  • eng

Conference Location

  • United States