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Heat-dependent opening of TRPV1 in the presence of capsaicin.

Publication ,  Journal Article
Kwon, DH; Zhang, F; Suo, Y; Bouvette, J; Borgnia, MJ; Lee, S-Y
Published in: Nat Struct Mol Biol
July 2021

Transient receptor potential vanilloid member 1 (TRPV1) is a Ca2+-permeable cation channel that serves as the primary heat and capsaicin sensor in humans. Using cryo-EM, we have determined the structures of apo and capsaicin-bound full-length rat TRPV1 reconstituted into lipid nanodiscs over a range of temperatures. This has allowed us to visualize the noxious heat-induced opening of TRPV1 in the presence of capsaicin. Notably, noxious heat-dependent TRPV1 opening comprises stepwise conformational transitions. Global conformational changes across multiple subdomains of TRPV1 are followed by the rearrangement of the outer pore, leading to gate opening. Solvent-accessible surface area analyses and functional studies suggest that a subset of residues form an interaction network that is directly involved in heat sensing. Our study provides a glimpse of the molecular principles underlying noxious physical and chemical stimuli sensing by TRPV1, which can be extended to other thermal sensing ion channels.

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Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

July 2021

Volume

28

Issue

7

Start / End Page

554 / 563

Location

United States

Related Subject Headings

  • Thermosensing
  • TRPV Cation Channels
  • Signal Transduction
  • Rats
  • Protein Domains
  • Protein Conformation
  • Protein Binding
  • Nanostructures
  • Humans
  • Hot Temperature
 

Citation

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Kwon, D. H., Zhang, F., Suo, Y., Bouvette, J., Borgnia, M. J., & Lee, S.-Y. (2021). Heat-dependent opening of TRPV1 in the presence of capsaicin. Nat Struct Mol Biol, 28(7), 554–563. https://doi.org/10.1038/s41594-021-00616-3
Kwon, Do Hoon, Feng Zhang, Yang Suo, Jonathan Bouvette, Mario J. Borgnia, and Seok-Yong Lee. “Heat-dependent opening of TRPV1 in the presence of capsaicin.Nat Struct Mol Biol 28, no. 7 (July 2021): 554–63. https://doi.org/10.1038/s41594-021-00616-3.
Kwon DH, Zhang F, Suo Y, Bouvette J, Borgnia MJ, Lee S-Y. Heat-dependent opening of TRPV1 in the presence of capsaicin. Nat Struct Mol Biol. 2021 Jul;28(7):554–63.
Kwon, Do Hoon, et al. “Heat-dependent opening of TRPV1 in the presence of capsaicin.Nat Struct Mol Biol, vol. 28, no. 7, July 2021, pp. 554–63. Pubmed, doi:10.1038/s41594-021-00616-3.
Kwon DH, Zhang F, Suo Y, Bouvette J, Borgnia MJ, Lee S-Y. Heat-dependent opening of TRPV1 in the presence of capsaicin. Nat Struct Mol Biol. 2021 Jul;28(7):554–563.

Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

July 2021

Volume

28

Issue

7

Start / End Page

554 / 563

Location

United States

Related Subject Headings

  • Thermosensing
  • TRPV Cation Channels
  • Signal Transduction
  • Rats
  • Protein Domains
  • Protein Conformation
  • Protein Binding
  • Nanostructures
  • Humans
  • Hot Temperature