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Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse.

Publication ,  Journal Article
Danilo, CA; Constantopoulos, E; McKee, LA; Chen, H; Regan, JA; Lipovka, Y; Lahtinen, S; Stenman, LK; Nguyen, T-VV; Doyle, KP; Slepian, MJ ...
Published in: Beneficial microbes
April 2017

There is a growing appreciation that our microbial environment in the gut plays a critical role in the maintenance of health and the pathogenesis of disease. Probiotic, beneficial gut microbes, administration can directly attenuate cardiac injury and post-myocardial infarction (MI) remodelling, yet the mechanisms of cardioprotection are unknown. We hypothesised that administration of Bifidobacterium animalis subsp. lactis 420 (B420), a probiotic with known anti-inflammatory properties, to mice will mitigate the pathological impact of MI, and that anti-inflammatory T regulatory (Treg) immune cells are necessary to impart protection against MI as a result of B420 administration. Wild-type male mice were administered B420, saline or Lactobacillus salivarius 33 (Ls-33) by gavage daily for 14 or 35 days, and underwent ischemia/reperfusion (I/R). Pretreatment with B420 for 10 or 28 days attenuated cardiac injury from I/R and reduced levels of inflammatory markers. Depletion of Treg cells by administration of anti-CD25 monoclonal antibodies eliminated B420-mediated cardio-protection. Further cytokine analysis revealed a shift from a pro-inflammatory to an anti-inflammatory environment in the probiotic treated post-MI hearts compared to controls. To summarise, B420 administration mitigates the pathological impact of MI. Next, we show that Treg immune cells are necessary to mediate B420-mediated protection against MI. Finally, we identify putative cellular, epigenetic and/or post-translational mechanisms of B420-mediated protection against MI.

Duke Scholars

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Published In

Beneficial microbes

DOI

EISSN

1876-2891

ISSN

1876-2883

Publication Date

April 2017

Volume

8

Issue

2

Start / End Page

257 / 269

Related Subject Headings

  • T-Lymphocytes, Regulatory
  • Probiotics
  • Myocardial Infarction
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Ligilactobacillus salivarius
  • Inflammation
  • Dietary Supplements
  • Cardiotonic Agents
 

Citation

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Danilo, C. A., Constantopoulos, E., McKee, L. A., Chen, H., Regan, J. A., Lipovka, Y., … Konhilas, J. P. (2017). Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse. Beneficial Microbes, 8(2), 257–269. https://doi.org/10.3920/bm2016.0119
Danilo, C. A., E. Constantopoulos, L. A. McKee, H. Chen, J. A. Regan, Y. Lipovka, S. Lahtinen, et al. “Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse.Beneficial Microbes 8, no. 2 (April 2017): 257–69. https://doi.org/10.3920/bm2016.0119.
Danilo CA, Constantopoulos E, McKee LA, Chen H, Regan JA, Lipovka Y, et al. Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse. Beneficial microbes. 2017 Apr;8(2):257–69.
Danilo, C. A., et al. “Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse.Beneficial Microbes, vol. 8, no. 2, Apr. 2017, pp. 257–69. Epmc, doi:10.3920/bm2016.0119.
Danilo CA, Constantopoulos E, McKee LA, Chen H, Regan JA, Lipovka Y, Lahtinen S, Stenman LK, Nguyen T-VV, Doyle KP, Slepian MJ, Khalpey ZI, Konhilas JP. Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse. Beneficial microbes. 2017 Apr;8(2):257–269.
Journal cover image

Published In

Beneficial microbes

DOI

EISSN

1876-2891

ISSN

1876-2883

Publication Date

April 2017

Volume

8

Issue

2

Start / End Page

257 / 269

Related Subject Headings

  • T-Lymphocytes, Regulatory
  • Probiotics
  • Myocardial Infarction
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Ligilactobacillus salivarius
  • Inflammation
  • Dietary Supplements
  • Cardiotonic Agents