Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse.

Journal Article (Journal Article)

There is a growing appreciation that our microbial environment in the gut plays a critical role in the maintenance of health and the pathogenesis of disease. Probiotic, beneficial gut microbes, administration can directly attenuate cardiac injury and post-myocardial infarction (MI) remodelling, yet the mechanisms of cardioprotection are unknown. We hypothesised that administration of Bifidobacterium animalis subsp. lactis 420 (B420), a probiotic with known anti-inflammatory properties, to mice will mitigate the pathological impact of MI, and that anti-inflammatory T regulatory (Treg ) immune cells are necessary to impart protection against MI as a result of B420 administration. Wild-type male mice were administered B420, saline or Lactobacillus salivarius 33 (Ls-33) by gavage daily for 14 or 35 days, and underwent ischemia/reperfusion (I/R). Pretreatment with B420 for 10 or 28 days attenuated cardiac injury from I/R and reduced levels of inflammatory markers. Depletion of Treg cells by administration of anti-CD25 monoclonal antibodies eliminated B420-mediated cardio-protection. Further cytokine analysis revealed a shift from a pro-inflammatory to an anti-inflammatory environment in the probiotic treated post-MI hearts compared to controls. To summarise, B420 administration mitigates the pathological impact of MI. Next, we show that Treg immune cells are necessary to mediate B420-mediated protection against MI. Finally, we identify putative cellular, epigenetic and/or post-translational mechanisms of B420-mediated protection against MI.

Full Text

Duke Authors

Cited Authors

  • Danilo, CA; Constantopoulos, E; McKee, LA; Chen, H; Regan, JA; Lipovka, Y; Lahtinen, S; Stenman, LK; Nguyen, T-VV; Doyle, KP; Slepian, MJ; Khalpey, ZI; Konhilas, JP

Published Date

  • April 2017

Published In

Volume / Issue

  • 8 / 2

Start / End Page

  • 257 - 269

PubMed ID

  • 28409534

Pubmed Central ID

  • PMC5815367

Electronic International Standard Serial Number (EISSN)

  • 1876-2891

International Standard Serial Number (ISSN)

  • 1876-2883

Digital Object Identifier (DOI)

  • 10.3920/bm2016.0119


  • eng