Diet and sex modify exercise and cardiac adaptation in the mouse.

Journal Article (Journal Article)

The heart adapts to exercise stimuli in a sex-dimorphic manner when mice are fed the traditional soy-based chow. Females undergo more voluntary exercise (4 wk) than males and exhibit more cardiac hypertrophy per kilometer run (18, 32). We have found that diet plays a critical role in cage wheel exercise and cardiac adaptation to the exercise stimulus in this sex dimorphism. Specifically, feeding male mice a casein-based, soy-free diet increases daily running distance over soy-fed counterparts to equal that of females. Moreover, casein-fed males have a greater capacity to increase their cardiac mass in response to exercise compared with soy-fed males. To further explore the biochemical mechanisms for these differences, we performed a candidate-based RT-PCR screen on genes previously implicated in diet- or exercise-based cardiac hypertrophy. Of the genes screened, many exhibit significant exercise, diet, or sex effects but only transforming growth factor-β1 shows a significant three-way interaction with no genes showing a two-way interaction. Finally, we show that the expression and activity of adenosine monophosphate-activated kinase-α2 and acetyl-CoA carboxylase is dependent on exercise, diet, and sex.

Full Text

Duke Authors

Cited Authors

  • Konhilas, JP; Chen, H; Luczak, E; McKee, LA; Regan, J; Watson, PA; Stauffer, BL; Khalpey, ZI; Mckinsey, TA; Horn, T; LaFleur, B; Leinwand, LA

Published Date

  • January 2015

Published In

Volume / Issue

  • 308 / 2

Start / End Page

  • H135 - H145

PubMed ID

  • 25398983

Pubmed Central ID

  • PMC4338936

Electronic International Standard Serial Number (EISSN)

  • 1522-1539

International Standard Serial Number (ISSN)

  • 0363-6135

Digital Object Identifier (DOI)

  • 10.1152/ajpheart.00532.2014


  • eng