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Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*.

Publication ,  Journal Article
Tan, X; Welsher, K
Published in: Angewandte Chemie (International ed. in English)
October 2021

Nanoparticles (NPs) adsorb proteins when exposed to biological fluids, forming a dynamic protein corona that affects their fate in biological environments. A comprehensive understanding of the protein corona is lacking due to the inability of current techniques to precisely measure the full corona in situ at the single-particle level. Herein, we introduce a 3D real-time single-particle tracking spectroscopy to "lock-on" to single freely diffusing polystyrene NPs and probe their individual protein coronas, primarily using bovine serum albumin (BSA) as a model system. The fluorescence signals and diffusive motions of the tracked NPs enable quantification of the "hard corona" using mean-squared displacement analysis. Critically, this method's particle-by-particle nature enabled a lock-in-type frequency filtering approach to extract the full protein corona, despite the typically confounding effect of high background signal from unbound proteins. From these results, the dynamic in situ full protein corona is observed to contain twice the number of proteins compared to the ex situ-measured "hard" protein corona.

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Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

October 2021

Volume

60

Issue

41

Start / End Page

22359 / 22367

Related Subject Headings

  • Single Molecule Imaging
  • Serum Albumin, Bovine
  • Protein Corona
  • Particle Size
  • Organic Chemistry
  • Nanoparticles
  • Models, Molecular
  • Cattle
  • Animals
  • 34 Chemical sciences
 

Citation

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Tan, X., & Welsher, K. (2021). Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*. Angewandte Chemie (International Ed. in English), 60(41), 22359–22367. https://doi.org/10.1002/anie.202105741
Tan, Xiaochen, and Kevin Welsher. “Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*.Angewandte Chemie (International Ed. in English) 60, no. 41 (October 2021): 22359–67. https://doi.org/10.1002/anie.202105741.
Tan X, Welsher K. Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*. Angewandte Chemie (International ed in English). 2021 Oct;60(41):22359–67.
Tan, Xiaochen, and Kevin Welsher. “Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*.Angewandte Chemie (International Ed. in English), vol. 60, no. 41, Oct. 2021, pp. 22359–67. Epmc, doi:10.1002/anie.202105741.
Tan X, Welsher K. Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*. Angewandte Chemie (International ed in English). 2021 Oct;60(41):22359–22367.
Journal cover image

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

October 2021

Volume

60

Issue

41

Start / End Page

22359 / 22367

Related Subject Headings

  • Single Molecule Imaging
  • Serum Albumin, Bovine
  • Protein Corona
  • Particle Size
  • Organic Chemistry
  • Nanoparticles
  • Models, Molecular
  • Cattle
  • Animals
  • 34 Chemical sciences