Plant "helper" immune receptors are Ca2+ -permeable nonselective cation channels.
Journal Article (Journal Article)
Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis , a subfamily of "helper" NLRs is required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca2+ ) influx in plant and human cells. NRG1.1-dependent Ca2+ influx and cell death were sensitive to Ca2+ channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca2+ influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that Arabidopsis helper NLRs form Ca2+ -permeable cation channels to directly regulate cytoplasmic Ca2+ levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly.
Full Text
Duke Authors
Cited Authors
- Jacob, P; Kim, NH; Wu, F; El-Kasmi, F; Chi, Y; Walton, WG; Furzer, OJ; Lietzan, AD; Sunil, S; Kempthorn, K; Redinbo, MR; Pei, Z-M; Wan, L; Dangl, JL
Published Date
- July 2021
Published In
Volume / Issue
- 373 / 6553
Start / End Page
- 420 - 425
PubMed ID
- 34140391
Pubmed Central ID
- PMC8939002
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.abg7917
Language
- eng