2+-permeable nonselective cation channels.">

Plant "helper" immune receptors are Ca2+ -permeable nonselective cation channels.

Journal Article (Journal Article)

Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis , a subfamily of "helper" NLRs is required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca2+ ) influx in plant and human cells. NRG1.1-dependent Ca2+ influx and cell death were sensitive to Ca2+ channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca2+ influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that Arabidopsis helper NLRs form Ca2+ -permeable cation channels to directly regulate cytoplasmic Ca2+ levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly.

Full Text

Duke Authors

Cited Authors

  • Jacob, P; Kim, NH; Wu, F; El-Kasmi, F; Chi, Y; Walton, WG; Furzer, OJ; Lietzan, AD; Sunil, S; Kempthorn, K; Redinbo, MR; Pei, Z-M; Wan, L; Dangl, JL

Published Date

  • July 2021

Published In

Volume / Issue

  • 373 / 6553

Start / End Page

  • 420 - 425

PubMed ID

  • 34140391

Pubmed Central ID

  • PMC8939002

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.abg7917

Language

  • eng