Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli .

Journal Article (Journal Article)

Autoinducible, two-stage protein expression leveraging phosphate-inducible promoters has been recently shown to enable not only high protein titers but also consistent performance across scales from screening systems (microtiter plates) to instrumented bioreactors. However, to date, small-scale production using microtiter plates and shake flasks relies on a complex autoinduction broth (AB) that requires making numerous media components, not all amenable to autoclaving. In this report, the authors develop a simpler media formulation (AB-2) with just a few autoclavable components. AB-2 is robust to small changes in its composition and performs equally, if not better, than AB across different scales. AB-2 will facilitate the adoption of phosphate-limited two-stage protein expression protocols.

Full Text

Duke Authors

Cited Authors

  • Menacho-Melgar, R; Hennigan, JN; Lynch, MD

Published Date

  • November 2021

Published In

Volume / Issue

  • 71 / 5

Start / End Page

  • 566 - 572

PubMed ID

  • 34431325

Electronic International Standard Serial Number (EISSN)

  • 1940-9818

International Standard Serial Number (ISSN)

  • 0736-6205

Digital Object Identifier (DOI)

  • 10.2144/btn-2021-0055

Language

  • eng