Mapping the genetic architecture of human traits to cell types in the kidney identifies mechanisms of disease and potential treatments.

Journal Article (Journal Article)

The functional interpretation of genome-wide association studies (GWAS) is challenging due to the cell-type-dependent influences of genetic variants. Here, we generated comprehensive maps of expression quantitative trait loci (eQTLs) for 659 microdissected human kidney samples and identified cell-type-eQTLs by mapping interactions between cell type abundances and genotypes. By partitioning heritability using stratified linkage disequilibrium score regression to integrate GWAS with single-cell RNA sequencing and single-nucleus assay for transposase-accessible chromatin with high-throughput sequencing data, we prioritized proximal tubules for kidney function and endothelial cells and distal tubule segments for blood pressure pathogenesis. Bayesian colocalization analysis nominated more than 200 genes for kidney function and hypertension. Our study clarifies the mechanism of commonly used antihypertensive and renal-protective drugs and identifies drug repurposing opportunities for kidney disease.

Full Text

Duke Authors

Cited Authors

  • Sheng, X; Guan, Y; Ma, Z; Wu, J; Liu, H; Qiu, C; Vitale, S; Miao, Z; Seasock, MJ; Palmer, M; Shin, MK; Duffin, KL; Pullen, SS; Edwards, TL; Hellwege, JN; Hung, AM; Li, M; Voight, BF; Coffman, TM; Brown, CD; Susztak, K

Published Date

  • September 2021

Published In

Volume / Issue

  • 53 / 9

Start / End Page

  • 1322 - 1333

PubMed ID

  • 34385711

Electronic International Standard Serial Number (EISSN)

  • 1546-1718

Digital Object Identifier (DOI)

  • 10.1038/s41588-021-00909-9

Language

  • eng

Conference Location

  • United States