Dorso-ventral heterogeneity in tracheal basal stem cells.

Journal Article (Journal Article)

The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs produce more, larger, clones than ventral BCs. Large clones were found between cartilage and smooth muscle where subpopulation of dorsal BCs exists. Three-dimensional organoid culture of BCs demonstrated that dorsal BCs show higher colony forming efficacy to ventral BCs. Gene ontology analysis revealed that genes expressed in dorsal BCs are enriched in wound healing while ventral BCs are enriched in response to external stimulus and immune response. Significantly, ventral BCs express Myostatin, which inhibits the growth of smooth muscle cells, and HGF, which facilitates cartilage repair. The results support the hypothesis that BCs from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.

Full Text

Duke Authors

Cited Authors

  • Tadokoro, T; Tanaka, K; Osakabe, S; Kato, M; Kobayashi, H; Hogan, BLM; Taniguchi, H

Published Date

  • September 15, 2021

Published In

Volume / Issue

  • 10 / 9

PubMed ID

  • 34396394

Pubmed Central ID

  • PMC8467549

Electronic International Standard Serial Number (EISSN)

  • 2046-6390

Digital Object Identifier (DOI)

  • 10.1242/bio.058676

Language

  • eng

Conference Location

  • England