A plant lipocalin promotes retinal-mediated oscillatory lateral root initiation.

Journal Article (Journal Article)

In Arabidopsis , de novo organogenesis of lateral roots is patterned by an oscillatory mechanism called the root clock, which is dependent on unidentified metabolites. To determine whether retinoids regulate the root clock, we used a chemical reporter for retinaldehyde (retinal)–binding proteins. We found that retinal binding precedes the root clock and predicts sites of lateral root organogenesis. Application of retinal increased root clock oscillations and promoted lateral root formation. Expression of an Arabidopsis protein with homology to vertebrate retinoid-binding proteins, TEMPERATURE INDUCED LIPOCALIN (TIL), oscillates in the region of retinal binding to the reporter, confers retinal-binding activity in a heterologous system, and, when mutated, decreases retinal sensitivity. These results demonstrate a role for retinal and its binding partner in lateral root organogenesis.

Full Text

Duke Authors

Cited Authors

  • Dickinson, AJ; Zhang, J; Luciano, M; Wachsman, G; Sandoval, E; Schnermann, M; Dinneny, JR; Benfey, PN

Published Date

  • September 2021

Published In

Volume / Issue

  • 373 / 6562

Start / End Page

  • 1532 - 1536

PubMed ID

  • 34446443

Pubmed Central ID

  • PMC8827267

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.abf7461

Language

  • eng