Multibow: digital spectral barcodes for cell tracing.

Journal Article (Journal Article)

We introduce a multicolor labeling strategy (Multibow) for cell tracing experiments in developmental and regenerative processes. Building on Brainbow-based approaches that produce colors by differential expression levels of different fluorescent proteins, Multibow adds a layer of label diversity by introducing a binary code in which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination. We have developed a library of constructs that contains seven different colors and three different subcellular localizations. Combining constructs from this library in the presence of Cre generates cells labeled with multiple independently expressed colors based on if each construct is ON or OFF following recombination. These labels form a unique "barcode" that allows the tracking of the cell and its clonal progenies in addition to expression level differences of each color. We tested Multibow in zebrafish which validates its design concept and suggests its utility for cell tracing applications in development and regeneration.

Full Text

Duke Authors

Cited Authors

  • Xiong, F; Obholzer, ND; Noche, RR; Megason, SG

Published Date

  • January 2015

Published In

Volume / Issue

  • 10 / 5

Start / End Page

  • e0127822 -

PubMed ID

  • 26010570

Pubmed Central ID

  • PMC4444339

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0127822

Language

  • eng