Skip to main content

Fibronectin Extra Domain A Promotes Liver Sinusoid Repair following Hepatectomy.

Publication ,  Journal Article
Sackey-Aboagye, B; Olsen, AL; Mukherjee, SM; Ventriglia, A; Yokosaki, Y; Greenbaum, LE; Lee, GY; Naga, H; Wells, RG
Published in: PloS one
January 2016

Liver sinusoidal endothelial cells (LSECs) are the main endothelial cells in the liver and are important for maintaining liver homeostasis as well as responding to injury. LSECs express cellular fibronectin containing the alternatively spliced extra domain A (EIIIA-cFN) and increase expression of this isoform after liver injury, although its function is not well understood. Here, we examined the role of EIIIA-cFN in liver regeneration following partial hepatectomy. We carried out two-thirds partial hepatectomies in mice lacking EIIIA-cFN and in their wild type littermates, studied liver endothelial cell adhesion on decellularized, EIIIA-cFN-containing matrices and investigated the role of cellular fibronectins in liver endothelial cell tubulogenesis. We found that liver weight recovery following hepatectomy was significantly delayed and that sinusoidal repair was impaired in EIIIA-cFN null mice, especially females, as was the lipid accumulation typical of the post-hepatectomy liver. In vitro, we found that liver endothelial cells were more adhesive to cell-deposited matrices containing the EIIIA domain and that cellular fibronectin enhanced tubulogenesis and vascular cord formation. The integrin α9β1, which specifically binds EIIIA-cFN, promoted tubulogenesis and adhesion of liver endothelial cells to EIIIA-cFN. Our findings identify a role for EIIIA-cFN in liver regeneration and tubulogenesis. We suggest that sinusoidal repair is enhanced by increased LSEC adhesion, which is mediated by EIIIA-cFN.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2016

Volume

11

Issue

10

Start / End Page

e0163737

Related Subject Headings

  • Wound Healing
  • Up-Regulation
  • Protein Isoforms
  • Protein Domains
  • Protein Binding
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Liver Regeneration
  • Liver
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sackey-Aboagye, B., Olsen, A. L., Mukherjee, S. M., Ventriglia, A., Yokosaki, Y., Greenbaum, L. E., … Wells, R. G. (2016). Fibronectin Extra Domain A Promotes Liver Sinusoid Repair following Hepatectomy. PloS One, 11(10), e0163737. https://doi.org/10.1371/journal.pone.0163737
Sackey-Aboagye, Bridget, Abby L. Olsen, Sarmistha M. Mukherjee, Alexander Ventriglia, Yasuyuki Yokosaki, Linda E. Greenbaum, Gi Yun Lee, Hani Naga, and Rebecca G. Wells. “Fibronectin Extra Domain A Promotes Liver Sinusoid Repair following Hepatectomy.PloS One 11, no. 10 (January 2016): e0163737. https://doi.org/10.1371/journal.pone.0163737.
Sackey-Aboagye B, Olsen AL, Mukherjee SM, Ventriglia A, Yokosaki Y, Greenbaum LE, et al. Fibronectin Extra Domain A Promotes Liver Sinusoid Repair following Hepatectomy. PloS one. 2016 Jan;11(10):e0163737.
Sackey-Aboagye, Bridget, et al. “Fibronectin Extra Domain A Promotes Liver Sinusoid Repair following Hepatectomy.PloS One, vol. 11, no. 10, Jan. 2016, p. e0163737. Epmc, doi:10.1371/journal.pone.0163737.
Sackey-Aboagye B, Olsen AL, Mukherjee SM, Ventriglia A, Yokosaki Y, Greenbaum LE, Lee GY, Naga H, Wells RG. Fibronectin Extra Domain A Promotes Liver Sinusoid Repair following Hepatectomy. PloS one. 2016 Jan;11(10):e0163737.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2016

Volume

11

Issue

10

Start / End Page

e0163737

Related Subject Headings

  • Wound Healing
  • Up-Regulation
  • Protein Isoforms
  • Protein Domains
  • Protein Binding
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Liver Regeneration
  • Liver