The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding.

Journal Article (Journal Article)

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus. Previous studies have indicated that the phosphodiester backbone is the main antigenic target, with electrostatic interactions important for high avidity. To define further these interactions, the effects of ionic strength on anti-DNA binding of SLE plasmas were assessed in association and dissociation assays by ELISA. As these studies demonstrated, increasing ionic strength to a concentration of 1000 mM NaCl reduced antibody binding although the extent of the reduction varied among samples. In dissociation assays, differences among plasmas were also observed. For one of the plasmas, binding to DNA displayed resistance to dissociation by increasing ionic strength even though these concentrations limited binding in association assays. Time course studies showed a gradual change in binding interactions. These studies indicate that anti-DNA binding can involve both electrostatic and non-electrostatic interactions, with binding in some plasmas showing evidence of hysteresis.

Full Text

Duke Authors

Cited Authors

  • Pisetsky, DS; Shaffer, R; Armstrong, DD; Spencer, DM

Published Date

  • October 2021

Published In

Volume / Issue

  • 231 /

Start / End Page

  • 108848 -

PubMed ID

  • 34492381

Pubmed Central ID

  • PMC8565600

Electronic International Standard Serial Number (EISSN)

  • 1521-7035

Digital Object Identifier (DOI)

  • 10.1016/j.clim.2021.108848


  • eng

Conference Location

  • United States