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Mapping Activity-Dependent Quasi-stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging.

Publication ,  Journal Article
Raja, SO; Chizhik, AI; Schmidt, CF; Enderlein, J; Ghosh, A
Published in: Nano letters
October 2021

Graphene-induced energy transfer (GIET) was recently introduced for sub-nanometric axial localization of fluorescent molecules. GIET relies on near-field energy transfer from an optically excited fluorophore to a single sheet of graphene. Recently, we demonstrated its potential by determining the distance between two leaflets of supported lipid bilayers. Here, we use GIET imaging for mapping quasi-stationary states of the inner and outer mitochondrial membranes before and during adenosine triphosphate (ATP) synthesis. We trigger the ATP synthesis state in vitro by activating mitochondria with precursor molecules. Our results demonstrate that the inner membrane approaches the outer membrane, while the outer membrane does not show any measurable change in average axial position upon activation. The inter-membrane space is reduced by ∼2 nm. This direct experimental observation of the subtle dynamics of mitochondrial membranes and the change in intermembrane distance upon activation is relevant for our understanding of mitochondrial function.

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Published In

Nano letters

DOI

EISSN

1530-6992

ISSN

1530-6984

Publication Date

October 2021

Volume

21

Issue

19

Start / End Page

8244 / 8249

Related Subject Headings

  • Nanoscience & Nanotechnology
  • Mitochondrial Membranes
  • Mitochondria
  • Lipid Bilayers
  • Graphite
  • Energy Transfer
 

Citation

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Raja, S. O., Chizhik, A. I., Schmidt, C. F., Enderlein, J., & Ghosh, A. (2021). Mapping Activity-Dependent Quasi-stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging. Nano Letters, 21(19), 8244–8249. https://doi.org/10.1021/acs.nanolett.1c02672
Raja, Sufi O., Alexey I. Chizhik, Christoph F. Schmidt, Jörg Enderlein, and Arindam Ghosh. “Mapping Activity-Dependent Quasi-stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging.Nano Letters 21, no. 19 (October 2021): 8244–49. https://doi.org/10.1021/acs.nanolett.1c02672.
Raja SO, Chizhik AI, Schmidt CF, Enderlein J, Ghosh A. Mapping Activity-Dependent Quasi-stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging. Nano letters. 2021 Oct;21(19):8244–9.
Raja, Sufi O., et al. “Mapping Activity-Dependent Quasi-stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging.Nano Letters, vol. 21, no. 19, Oct. 2021, pp. 8244–49. Epmc, doi:10.1021/acs.nanolett.1c02672.
Raja SO, Chizhik AI, Schmidt CF, Enderlein J, Ghosh A. Mapping Activity-Dependent Quasi-stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging. Nano letters. 2021 Oct;21(19):8244–8249.
Journal cover image

Published In

Nano letters

DOI

EISSN

1530-6992

ISSN

1530-6984

Publication Date

October 2021

Volume

21

Issue

19

Start / End Page

8244 / 8249

Related Subject Headings

  • Nanoscience & Nanotechnology
  • Mitochondrial Membranes
  • Mitochondria
  • Lipid Bilayers
  • Graphite
  • Energy Transfer