Refraining from use diminishes cannabis-associated epigenetic changes in human sperm.

Journal Article (Journal Article)

Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline and again following a 77-day period of cannabis abstinence (one spermatogenic cycle). Users and controls did not significantly differ by demographics or semen analyses. Whole-genome bisulfite sequencing identified 163 CpG sites with significantly different DNA methylation in sperm between groups (P < 2.94 × 10-9). Genes associated with altered CpG sites were enriched with those involved in development, including cardiogenesis and neurodevelopment. Many of the differences in sperm DNA methylation between groups were diminished after cannabis abstinence. These results indicate that sustained cannabis abstinence significantly reduces the number of sperm showing cannabis-associated alterations at genes important for early development.

Full Text

Duke Authors

Cited Authors

  • Schrott, R; Murphy, SK; Modliszewski, JL; King, DE; Hill, B; Itchon-Ramos, N; Raburn, D; Price, T; Levin, ED; Vandrey, R; Corcoran, DL; Kollins, SH; Mitchell, JT

Published Date

  • 2021

Published In

Volume / Issue

  • 7 / 1

Start / End Page

  • dvab009 -

PubMed ID

  • 34557312

Pubmed Central ID

  • PMC8455898

Electronic International Standard Serial Number (EISSN)

  • 2058-5888

Digital Object Identifier (DOI)

  • 10.1093/eep/dvab009


  • eng

Conference Location

  • England