Mast cell function is not altered by Coronin-1A deficiency.

Journal Article (Journal Article)

Coronin-1A is a WD repeat protein family member, highly expressed in all hematopoietic lineages, and acts as a regulator of F-actin dynamics and Ca2+ signaling. In Coro1a(Lmb3) mice results in inactivation of the protein and leads to disease resistance in a model of lupus erythematosus. In Coro1a(-/-) and Coro1a(Lmb3) mice, peripheral T cells exhibit impairments in survival, migration, activation, and Ca2+ flux. In this study, we show that in vitro-differentiated mast cells from Coro1a(Lmb3) mice are viable, developed normally, and are fully functional in assays of degranulation, cytokine secretion, and chemotactic migration, despite increased F-actin levels. In Coro1a(Lmb3) mast cells, Ca2+ flux in response to physiological FcεRI stimulation is unaffected. Finally, Coro1a(Lmb3) mice showed similar in vivo mast cell responses as the WT mice. Coronin-1B and Coronin-1C expression levels were not increased in Coro1a(Lmb3) mast cells but were higher in mast cells than in CD4 T cells or B cells in WT mice. We conclude that Coronin-1A activity is not required for mast cell function.

Full Text

Duke Authors

Cited Authors

  • Arandjelovic, S; Wickramarachchi, D; Hemmers, S; Leming, SS; Kono, DH; Mowen, KA

Published Date

  • October 2010

Published In

Volume / Issue

  • 88 / 4

Start / End Page

  • 737 - 745

PubMed ID

  • 20643816

Pubmed Central ID

  • PMC2974433

Electronic International Standard Serial Number (EISSN)

  • 1938-3673

Digital Object Identifier (DOI)

  • 10.1189/jlb.0310131

Language

  • eng

Conference Location

  • United States