Detection of necrosis by release of lactate dehydrogenase activity.

Journal Article (Journal Article)

Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.

Full Text

Duke Authors

Cited Authors

  • Chan, FK-M; Moriwaki, K; De Rosa, MJ

Published Date

  • 2013

Published In

Volume / Issue

  • 979 /

Start / End Page

  • 65 - 70

PubMed ID

  • 23397389

Pubmed Central ID

  • PMC3763497

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-290-2_7

Language

  • eng

Conference Location

  • United States