SARS-CoV-2 triggers DNA damage response in Vero E6 cells.

Journal Article (Journal Article)

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for the current COVID-19 pandemic and has now infected more than 200 million people with more than 4 million deaths globally. Recent data suggest that symptoms and general malaise may continue long after the infection has ended in recovered patients, suggesting that SARS-CoV-2 infection has profound consequences in the host cells. Here we report that SARS-CoV-2 infection can trigger a DNA damage response (DDR) in African green monkey kidney cells (Vero E6). We observed a transcriptional upregulation of the Ataxia telangiectasia and Rad3 related protein (ATR) in infected cells. In addition, we observed enhanced phosphorylation of CHK1, a downstream effector of the ATR DNA damage response, as well as H2AX. Strikingly, SARS-CoV-2 infection lowered the expression of TRF2 shelterin-protein complex, and reduced telomere lengths in infected Vero E6 cells. Thus, our observations suggest SARS-CoV-2 may have pathological consequences to host cells beyond evoking an immunopathogenic immune response.

Full Text

Duke Authors

Cited Authors

  • Victor, J; Deutsch, J; Whitaker, A; Lamkin, EN; March, A; Zhou, P; Botten, JW; Chatterjee, N

Published Date

  • November 19, 2021

Published In

Volume / Issue

  • 579 /

Start / End Page

  • 141 - 145

PubMed ID

  • 34600299

Pubmed Central ID

  • PMC8440005

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2021.09.024

Language

  • eng

Conference Location

  • United States